塔里木河下游胡杨根际土壤细菌群落多样性分析

doi:10.12118/j.issn.1000–6060.2021.03.17

Abstract

Abstract:

Rhizosphere bacteria play an essential role in promoting plant growth and protecting plant health. In this study, we use the high throughput sequencing to analyze rhizosphere bacteria at different developmental periods of Populus euphratica (the four periods are young, medium, overripe, and decline periods) in the lower reaches of Tarim River in Xinjiang, China. Canonical correspondence and Spearman correlation analyses were used to investigate the correlation between bacterial community and environmental factors. The results showed that (1) out of the soil samples collected from the soils under the four developmental periods of P. euphratica, 98028 effective sequences and 7287 operational taxonomic units (OTUs) were obtained, the OTUs numbers of the four periods were 3701, 4543, 4297, and 3710, respectively. From comparative identification, 73 phyla, 165 classes, 339 orders, 454 families, 651 genera, and 205 species were obtained with the development of P. euphratica. (2) Alpha diversity analysis showed that the bacterial community diversity was the highest at the overripe period (Shannon and Simpson index), the bacterial community abundance was the highest at the medium period (Chao1 and ACE index), the abundance and diversity of the rhizosphere bacterial community showed a trend of first increasing and then decreasing; however, there was no significant difference in bacterial abundance and diversity in rhizosphere soil at different developmental periods. (3) Proteobacteria, unidentified_Bacteria, and Halobacterota were the dominant bacteria at the phylum level in the rhizosphere of P. euphratica, whereas Marinobacter, Halomonas, and Woeseia were the dominant bacteria at the genus level. Compared with the phylum level, the bacterial community composition of soil samples was significantly different at the genus level. The dominant bacterial genera of rhizosphere bacterial community of P. euphratica were different in different developmental periods; Marinobacter, Halomonas, and Woeseia were, respectively, the optimal bacteria genus in the young, medium/overripe, and decline periods. (4) As shown in the cluster analysis, the bacterial communities of all soils were divided into two groups: the medium and decline periods were clustered into one group, and the young and overripe periods were clustered into another group. (5) Canonical correspondence analysis showed that the soil water content, total potassium, total salt, and soil pH were the main environmental factors influencing the bacterial community composition in the rhizosphere soil of P. euphratica (P<0.05). The results revealed the composition of rhizosphere bacterial communities in different periods of P. euphratica and the main environmental factors affecting the community composition. The findings in this study may provide a scientific basis for the study of rhizosphere microorganisms and the interaction between plants and microorganisms in arid areas.

Key words: rhizosphere, soil microorganism, high throughput sequencing, bacterial community, Tarim River

LI Yuanyuan,PENG Mengwen,DANG Hanli,JIANG Meng,ZHUANG Li,LI Guifang. Bacterial communities diversity of Populus euphratica rhizospheric soil in the lower reaches of Tarim River[J].Arid Land Geography, 2021, 44(3): 750-758.

Figures/Tables 11

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References 31

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生长时期	胸径/cm	树高/m	冠幅/m×m
幼龄期（A）	3.50~5.41	2.53~3.25	2.02×1.93~2.62×3.13
中壮期（B）	8.12~9.62	4.50~5.50	2.82×3.20~3.50×3.98
过熟期（C）	59.23~67.88	12.30~16.50	6.21×6.09~5.86×7.40
衰亡期（D）	枯立木

土壤理化性质	幼龄期(A)	中壮期(B)	过熟期(C)	衰亡期(D)	裸地(CK)
含水量（SWC）/%	6.34±0.65a	3.41±1.11bc	3.17±1.05bc	5.02±0.24ab	1.61±0.09c
有机质（OM）/g·kg^-1	7.06±1.66ab	7.17±2.13ab	5.66±1.11b	11.04±1.44a	5.16±0.78b
全氮（TN）/g·kg^-1	0.42±0.07ab	0.51±0.03ab	0.37±0.04b	0.54±0.02a	0.41±0.02ab
全磷（TP）/g·kg^-1	0.58±0.01a	0.61±0.03a	0.61±0.05a	0.64±0.01a	0.65±0.03a
全钾（TK）/g·kg^-1	18.25±0.54b	17.54±0.18bc	17.93±0.23b	19.71±0.15a	16.85±0.23c
硝态氮（NO₃^-）/mg·kg^-1	4.86±1.04ab	3.16±2.19b	2.84±0.87b	3.97±0.43ab	8.14±2.35a
铵态氮（NH₄⁺）/mg·kg^-1	2.49±0.04a	2.94±0.41a	2.46±0.69a	2.11±0.31a	2.72±0.21a
速效磷（AP）/mg·kg^-1	3.06±1.83ab	2.01±1.45b	1.86±1.09b	6.03±0.93a	2.31±0.71ab
速效钾（AK）/mg·kg^-1	421.81±35.05c	345.13±118.76c	931.10±215.86b	3398.67±235.91a	389.78±81.09c
pH	7.78±0.05c	8.43±0.13b	8.61±0.15b	9.49±0.19a	7.89±0.19c
电导率（EC）/mS·cm^-1	2.78±0.15b	2.11±1.46b	2.74±1.08b	7.96±1.14a	5.34±0.78ab
总盐（TDS）/g·kg^-1	9.78±0.42b	7.81±5.34b	8.65±3.88b	30.01±4.59a	18.84±2.98ab

生长时期	Shannon指数	Simpson指数	Chao1指数	ACE指数	覆盖率
裸地（CK）	8.23±0.39a	0.9840±0.0052a	2724.56±211.81a	2850.77±240.89a	0.9887±0.0006
幼龄期（A）	8.18±0.76a	0.9817±0.0157a	2536.04±181.46a	2425.85±420.43a	0.9903±0.0006
中壮期（B）	8.83±0.70a	0.9917±0.0038a	3003.81±509.22a	3136.30±534.48a	0.9873±0.0012
过熟期（C）	8.86±0.40a	0.9920±0.0017a	2729.57±277.58a	2790.52±240.48a	0.9897±0.0006
衰亡期（D）	7.85±0.90a	0.9753±0.0201a	2549.67±249.84a	2653.46±253.02a	0.9890±0.0010
P值	0.324	0.419	0.398	0.250

Bacterial communities diversity of Populus euphratica rhizospheric soil in the lower reaches of Tarim River

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