接种摩西球囊霉胡杨幼株对渐进式土壤水分亏缺的响应

摘要/Abstract

摘要： 通过采用室外盆栽试验，对2 a生胡杨实生幼株接种摩西球囊霉菌剂后对土壤渐进式水分亏缺的气体交换、光合作用和生长响应进行了分析，结果表明：在饱和灌溉6 d后，盆栽胡杨幼株试验的土壤0～40 cm范围平均含水量就从田间持水量下降至轻度干旱胁迫水平，第15 d后下降至重度干旱胁迫水平。在不同停灌时期，接种摩西球囊霉菌剂胡杨幼株生理活动对土壤水分亏缺的响应与未接种株的不同。其中：停灌3 d后，叶片气孔导度的日变化趋势在接种与未接种株间相似，且无显著差异；停灌15 d后，接种株叶片气孔导度的日变化趋势与未接种株的不同，接种株的比未接种株的高12～131 mol·m-2·s-1。在不同停灌时期，接种株叶片光系统II（PSⅡ）的实际量子产量日变化趋势与未接种株的相似，但日变幅不同，其中，停灌6 d后，接种与未接种株日变幅分别为0.42、0.48，停灌15 d后则分别为0.45、0.51。接种株叶片光系统II（PSⅡ）的光化学猝灭系数日变化趋势与未接种株的相似，其中，停灌6 d后两者间的差异不显著，停灌15 d后，除了8∶00和18∶00外，其它时间接种株的显著高于未接种株的[（P]<0.05）。在水分渐进式亏缺期内，接种株有新的侧枝长出，未接种株没有，接种胡杨幼株的高度、基径的增幅均比未接种株的大。

关键词: 丛枝菌根, 水分亏缺, 胁迫, 响应

Abstract: In this study，pot experiments were conducted to investigate whether arbuscular mycorrhizal fungi （Glomus mosseae） influenced physiology of Populus euphratica seedlings under a short-term soil water deficit in an extreme arid area. Two-year-old native P. euphratica seedlings inoculated with 20 ml of Glomus mosseae inoculum，a mixture of spores，mycorrhizal root fragments，and rhizospheric soil（Spore density was 50 spores /ml）. Non-inoculated seedlings were put under water deficit treatments at outdoor for a period of 21 days in the lower reaches of Tarim River. The water deficit treatments were imposed by withholding irrigation. The results showed that soil water deficit affected the physiology of Populus euphratica seedlings. However，compared with the non-inoculated seedlings under severe soil water deficit，the inoculated seedlings had greater stomatal conductance，actual photochemical quantum yield of PSII，and photochemistry quenching. After 6 and 15 days of water withholding，the daily changing trend in actual quantum yield of photosystem II （PSII） was similar between the inoculated and non-inoculated groups but not the daily changing ranges. The daily changing ranges of actual quantum yield in the inoculated group were 0.42 and 0.45 while those in the non-inoculated group were 0.48 and 0.51 after 6 and 15 days respectively. The photochemical quenching coefficients in the inoculated group were 0.08-0.11 which was higher than those in the non-inoculated group. During the progressive water deficit，inoculated seedlings had new lateral branches grow out but not the non-inoculated seedlings. The increases of the height and base diameter of the inoculated seedlings were more than those of the non-inoculated seedlings. This study shed a light on the mechanisms of stress-resistance of the plant in arid regions and could provide scientific basis for fully utilizing the Glomus mosseae resources to improve the protection of Populus euphratica from drought stresses in arid regions.

Key words: arbuscular mycorrhizal, water deficit, stress, response

中图分类号:

Q948.113

杨玉海，李卫红，陈亚宁，朱成刚，马建新. 接种摩西球囊霉胡杨幼株对渐进式土壤水分亏缺的响应[J]. 干旱区地理, 2015, 38(1): 60-66.

YANG Yu-hai，LI Wei-hong，CHEN Ya-ning，ZHU Chen-gang，MA Jian-xin. Response of Populus euphratica seedling inoculated Glomus mosseae to progressive soil water deficit[J]. , 2015, 38(1): 60-66.

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