羽毛针禾(Stipagrostis pennata)根鞘暗色丝孢菌降解功能的初步探究

Abstract

Abstract: Stipagrostis pennata plant mainly distributed in Gurbantunggut Desert of the Junggar basin in the northern part of Tianshan Mountains,Xinjiang,China. It has an excellent drought resistance and sand fixation due to the special structure of rhizosheath. The present study for rhizosheath microorganism mainly concentrated in the nitrogen fixation strains,while the fungi function is poorly understood. Previous research has laid the foundation for the study of dematiaceous hyphomycetes from stipagrostis pennata rhizosheath,but their function and ecological significance still need further systematic research. Therefore,in this study,five dematiaceous hyphomycetes,which isolated from the rhizosheaths of S. pennata were chosen due to their strong stress resistance to investigate the production of cellulase enzyme and the ability of degradation of polyethylene. The results show that all selected strains except 0103 had the ability to produce cellulase. Strain 0104 showed the highest cellulase production by showing a clear halo zone around the colony on the selective medium,and the hydrolysis ability was as high as 1.70,followed by strains 0304,0105,and 0204. Moreover,the filter paper degradation and weight loss method confirmed the ability of strain 0104 for cellulose degradation,because the paper weight loss rate reached 55.72%. Cultivate five strains with polyethylene as the sole carbon source solid medium. strains 0103 was found to well degrade polyethylene. The cavity size produced by degradation of polyethylene was observed under the light microscope,and measure its size with Digimizer(3.75 mm²). Thus,0104 and 0103 strains showed potential in the degradation of cellulose and polyethylene. The functional fungi in the rhizosheath of S. pennata needs to be further developed and utilized.

Key words: Stipagrostis pennata, rhizosheath, dematiaceous hyphomycetes, degradation

CLC Number:

S182

CHEN Jie-zuo, WANG Hui-ming, GUO Jian-wei, HU Wen-ge, WU Nan, ZHANG Yuan-ming. Degradation function of dematiaceous hyphomycetes isolated from rhizosheath of desert plant Stipagrostis pennata[J]., 2017, 40(4): 817-823.

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Degradation function of dematiaceous hyphomycetes isolated from rhizosheath of desert plant Stipagrostis pennata

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