天山北部不同生境苜蓿根际土壤真菌分布特征及驱动因子

doi:10.12118/j.issn.1000-6060.2023.499

Abstract

Abstract:

In agricultural ecosystems, rhizosphere microbial communities serve as the driving force for the successful cycling and transformation of nutrients and organic matter in soil, exerting a significant impact on the growth, health, and nutritional status of crops. To investigate the diversity of rhizosphere fungi in different habitats in the northern Tianshan Mountains and the driving factors behind their variations, this study employed high-throughput sequencing to analyze the diversity, structures, functions, and driving factors of rhizosphere and non-rhizosphere fungi in alfalfa rhizosphere soils in mountainous and plain habitats. The results revealed that: (1) Soil nitrogen, potassium, organic matter, and enzyme activity in the mountainous alfalfa rhizosphere soil were significantly higher than those in plain alfalfa rhizosphere soil, while electrical conductivity and pH were significantly lower than those in plain alfalfa rhizosphere soil. (2) Significant differences were observed in the fungal communities in mountainous and plain soils, with the operational taxonomic units (OTUs), Chao1, and ACE indices of plain alfalfa rhizosphere soil being significantly higher than those of mountainous alfalfa rhizosphere soil; additionally, the OTUs, Chao1, ACE, and Shannon indices showed significantly higher values in rhizosphere soils than those in non-rhizosphere soils. (3) The dominant fungal phyla in the region were Ascomycota, Mortierellomycota, Basidiomycota, and Blastocladiomycota, with notable differences in their distribution across different habitats. (4) Based on FUNGuild’s fungal functional predictions, pathogenic fungi in mountainous areas were significantly higher than those in plain areas, while arbuscular mycorrhizal fungi were significantly lower in mountainous areas. (5) Redundancy analysis and Mantel tests indicated that soil pH, total nitrogen, total potassium and available potassium were the main driving factors for the differential distribution of fungal community structures in soils.

Key words: rhizosphere microecology, functional prediction, high-throughput sequencing, fungal diversity, northern Tianshan Mountains

LI Man, DENG Qizhang, JING Yuting, WU Yong, ZHANG Chengming, XIA Shan, ZHAI Yaping, YANG Pan, LIU Hongling, ZHUANG Li. Distribution characteristics and driving factors of soil fungi between alfalfa roots in different habitats in northern Tianshan Mountains[J].Arid Land Geography, 2024, 47(7): 1187-1198.

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理化因子	重要性排名	解释率/%	F值	P值
全氮	1	88.4	75.9	0.001
pH	2	83.2	49.4	0.002
电导率	3	76.7	33.0	0.001
速效钾	4	61.4	15.9	0.004
全钾	5	53.5	11.5	0.008
速效磷	6	9.5	1.0	0.296
全磷	7	1.5	0.2	0.733
含水率	8	1.2	0.1	0.781

Distribution characteristics and driving factors of soil fungi between alfalfa roots in different habitats in northern Tianshan Mountains

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