干旱风沙区植被重建初期对土壤微生物群落结构的影响

doi:10.12118/j.issn.1000-6060.2022.055

Abstract

Abstract:

To elucidate the effect of revegetation on soil microbial community structure in an arid desert region, the revegetation area built to protect the Wuhai-Maqin Highway across the Tengger Desert, northwest China from dune blow was selected as the study area. High-throughput sequencing technology was applied to reveal the characteristics of soil microbial community structure under the revegetation, and selecting the moving dune as a comparison. Results found that microbial community composition was similar between the revegetated soil and moving dune, dominated by Actinobacteria (58.53%-67.85%) and Proteobacteria (16.53%-19.68%). Nocardia, Methylotenera, Rubellimicrobium, and Microvirga were the dominant genera in the revegetation area and moving dune. In contrast to the moving dune, the revegetation significantly increased the relative abundance and diversity of most phylum of microorganisms but significantly decreased soil pH, electrical conductivity, and salinity. Furthermore, compared with the moving dune, the revegetation significantly increased soil microbial biomass P and C:N at three soil layers, including 0-5 cm, 5-10 cm, and 10-20 cm. Overall, the revegetation could significantly improve the nutrient level and the relative abundance and diversity of soil microorganisms at the topsil layer of the desert ecological protection belt beside the highway.

Key words: arid windy sand area, vegetation reconstruction, microbial community structure

ZHANG Libin, HE Mingzhu, ZHANG Kecun, AN Zhishan, WANG Jinguo, HUI Yingxin, JIA Xiaolong. Effect of preliminary vegetation reconstruction on soil microorganism community structure in arid desert area[J].Arid Land Geography, 2022, 45(6): 1916-1926.

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菌属	植被重建区/%	流沙区/%
异常球菌属（Deinococcus）	0.21	2.44
栖水菌属（Enhydrobacter）	0.04	0.72
烟草黄色土源菌属（Flavisolibacter）	0.44	1.00
微红微球菌属（Rubellimicrobium）	1.02	0.80
甲基柔膜菌属（Methylotenera）	1.24	0.81
壤霉菌属（Agromyces）	1.00	0.41
微枝型杆菌属（Microvirga）	1.32	0.72
根瘤菌属（Rhizobium）	0.85	0.01
大理石雕菌属（Marmoricola）	1.27	0.41
诺卡氏菌属（Nocardioides）	7.19	1.33

区域	土层/cm	平均长度/bp	物种数	距离	覆盖度	Chao1指数	Ace指数	Simpson指数	Shannon指数
植被重建区	0~5	411	1815	0.03	0.985	2886	2423	0.980	7.878
	5~10	409	1576	0.03	0.990	1812	1876	0.734	5.033
	10~20	411	1589	0.03	0.992	1750	1778	0.865	6.248
	20~50	411	1073	0.03	0.991	1451	1434	0.744	4.041
	50~100	415	1956	0.03	0.991	2093	2177	0.960	8.100
	平均	411	1602	0.03	0.990	1998	1938	0.857	6.260
流沙区	0~5	412	1908	0.03	0.991	2101	2137	0.986	8.207
	5~10	413	1547	0.03	0.991	1758	1807	0.893	6.212
	10~20	410	1164	0.03	0.988	1730	1675	0.352	2.309
	20~50	410	1362	0.03	0.991	1533	1644	0.549	3.599
	50~100	410	1398	0.03	0.988	1771	1830	0.521	3.330
	平均	411	1476	0.03	0.990	1779	1819	0.660	4.731

区域	土层/cm	pH	EC/μS·cm^-1	Salt/ng·L^-1	MBP/mg·kg^-1	MBC/mg·kg^-1	MBN/mg·kg^-1	MBC:MBN	Eh/mV
植被重建区	0~5	8.21	40.80	0.20	0.18	85.47	0.95	89.95	-63.1
	5~10	8.15	43.00	0.20	0.26	144.19	0.92	156.92	-78.0
	10~20	8.19	41.70	0.20	0.27	392.87	1.16	338.38	-84.0
	20~50	8.13	45.20	0.20	0.03	25.26	1.74	14.54	-89.0
	50~100	8.16	41.80	0.20	0.20	583.18	2.32	251.83	-95.9
流沙区	0~5	7.92	79.60	0.40	0.13	11.19	2.74	4.09	-67.5
	5~10	8.43	78.80	0.40	0.14	63.84	2.32	27.54	-66.2
	10~20	8.41	81.70	0.40	0.05	12.02	1.97	6.09	-66.2
	20~50	8.53	78.30	0.30	0.37	13.68	1.89	7.25	-63.7
	50~100	8.69	74.30	0.30	0.20	90.00	1.35	66.70	-64.0

Effect of preliminary vegetation reconstruction on soil microorganism community structure in arid desert area

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