干旱风沙区植被重建初期对土壤微生物群落结构的影响

doi:10.12118/j.issn.1000-6060.2022.055

Abstract

Abstract:

To elucidate the effect of revegetation on soil microbial community structure in an arid desert region, the revegetation area built to protect the Wuhai-Maqin Highway across the Tengger Desert, northwest China from dune blow was selected as the study area. High-throughput sequencing technology was applied to reveal the characteristics of soil microbial community structure under the revegetation, and selecting the moving dune as a comparison. Results found that microbial community composition was similar between the revegetated soil and moving dune, dominated by Actinobacteria (58.53%-67.85%) and Proteobacteria (16.53%-19.68%). Nocardia, Methylotenera, Rubellimicrobium, and Microvirga were the dominant genera in the revegetation area and moving dune. In contrast to the moving dune, the revegetation significantly increased the relative abundance and diversity of most phylum of microorganisms but significantly decreased soil pH, electrical conductivity, and salinity. Furthermore, compared with the moving dune, the revegetation significantly increased soil microbial biomass P and C:N at three soil layers, including 0-5 cm, 5-10 cm, and 10-20 cm. Overall, the revegetation could significantly improve the nutrient level and the relative abundance and diversity of soil microorganisms at the topsil layer of the desert ecological protection belt beside the highway.

Key words: arid windy sand area, vegetation reconstruction, microbial community structure

ZHANG Libin, HE Mingzhu, ZHANG Kecun, AN Zhishan, WANG Jinguo, HUI Yingxin, JIA Xiaolong. Effect of preliminary vegetation reconstruction on soil microorganism community structure in arid desert area[J].Arid Land Geography, 2022, 45(6): 1916-1926.

Figures/Tables 10

Fig. 1

Tab. 1

Fig. 2

Fig. 3

Tab. 2

Fig. 4

Fig. 5

Fig. 6

Tab. 3

Fig. 7

References 33

[1]	马晓俊, 李云飞. 腾格里沙漠东南缘植被恢复过程中土壤微生物量及酶活性[J]. 中国沙漠, 2019, 39(6): 159-166.
	[Ma Xiaojun, Li Yunfei. Soil microbial biomass and enzyme activities during revegetation process in the southeastern frige of the Tengger Desert[J]. Journal of Desert Research, 2019, 39(6): 159-166.]
[2]	刘世荣. 荒漠化地区植被恢复与重建的生态对策[C]// 周光召. 全面建设小康社会:中国科技工作者的历史责任——中国科协2003年学术年会论文集(上). 北京: 中国科学技术出版社, 2003: 182-183.
	[Liu Shirong. Ecological countermeasures for vegetation restoration and reconstruction in desertification areas[C]// Zhou Guangzhao. Building a Well-off Society in an All-round Way: The Historical Responsibility of Chinese Scientists and Technologists:Proceedings of 2003 Annual Conference of China Association for Science and Technology (I). Beijing: Science and Technology of China Press, 2003: 182-183.]
[3]	张燕燕, 曲来叶, 陈利顶, 等. 黄土丘陵沟壑区不同植被类型土壤微生物特性[J]. 应用生态学报, 2010, 21(1): 165-173. pmid: 20387439
	[Zhang Yanyan, Qu Laiye, Chen Liding, et al. Soil microbial properties under different vegetation types in Loess hilly region[J]. Chinese Journal of Applied Ecology, 2010, 21(1): 165-173.] pmid: 20387439
[4]	李晓娜, 王超, 张微微, 等. 京郊荒滩地短期植被恢复对土壤理化性质及微生物群落结构的影响[J]. 水土保持学报, 2019, 33(5): 343-348, 357.
	[Li Xiaona, Wang Chao, Zhang Weiwei, et al. Effects of short-term vegetation restoration on soil physicochemical characteristics and microbial community structures in the wasteland of Beijing suburb[J]. Journal of Soil and Water Conservation, 2019, 33(5): 343-348, 357.]
[5]	石万里, 王辉, 马维伟. 沙区植被恢复对土壤微生物量及活性的影响[J]. 中国沙漠, 2017, 37(3): 507-513.
	[Shi Wanli, Wang Hui, Ma Weiwei. Effects of vegetation restoration on soil microbial biomass and activity in desert area[J]. Journal of Desert Research, 2017, 37(3): 507-513.]
[6]	张振佳, 曹银贵, 王舒菲, 等. 平朔黄土露天矿区复垦地表层土壤微生物与酶活性分析[J]. 生态学报, 2021, 41(1): 110-123.
	[Zhang Zhenjia, Cao Yingui, Wang Shufei, et al. Characteristics and differences of surface soil microbial population and enzyme activities in opencast mining area of Pingshuo[J]. Acta Ecologica Sinica, 2021, 41(1): 110-123.]
[7]	韦应莉, 曹文侠, 李建宏, 等. 不同放牧与围封高寒灌丛草地土壤微生物群落结构PLFA分析[J]. 生态学报, 2018, 38(13): 4897-4908.
	[Wei Yingli, Cao Wenxia, Li Jianhong, et al. Phospholipidfatty acid (PLFA) analysis of soil microbial community structure with different intensities of of grazing and fencing in alpine shrubland[J]. Acta Ecologica Sinica, 2018, 38(13): 4897-4908.]
[8]	马欣, 罗珠珠, 张耀全, 等. 黄土高原雨养区不同种植年限紫花苜蓿土壤细菌群落特征与生态功能预测[J]. 草业学报, 2021, 30(3): 54-67.
	[Ma Xin, Luo Zhuzhu, Zhang Yaoquan, et al. Distribution characteristics and ecological function predictions of soil bacterial communities in rainfed alfalfa fields on the Loess Plateau[J]. Acta Prataculturae Sinica, 2021, 30(3): 54-67.]
[9]	张超, 马亚亚, 刘国彬, 等. 黄土高原植被恢复过程中土壤微生物的演变特征[J]. 水土保持通报, 2018, 38(6): 240-247.
	[Zhang Chao, Ma Yaya, Liu Guobin, et al. Evolution of soil microbial community during vegetation restoration in Loess Plateau[J]. Bulletin of Soil and Water Conservation, 2018, 38(6): 240-247.]
[10]	张海芳. 呼伦贝尔沙地不同植被恢复模式土壤微生物多样性[D]. 天津: 天津师范大学, 2012.
	[Zhang Haifang. Soil microbial diversity under different vegetation restoration modes in Hulun Buir Sandy Land[D]. Tianjin: Tianjin Normal University, 2012.]
[11]	周智彬, 李培军. 塔克拉玛干沙漠腹地人工绿地土壤中微生物的生态分布及其与土壤因子间的关系[J]. 应用生态学报, 2003, 14(8): 1246-1250. pmid: 14655351
	[Zhou Zhibin, Li Peijun. Ecological distribution of soil microorganism in artificial greenbelt in hinterland of Takilimakan Desert and their relations with soil factors[J]. Chinese Journal of Applied Ecology, 2003, 14(8): 1246-1250.] pmid: 14655351
[12]	卢小妹. 民勤人工梭梭林土壤微生物时空异质性与土壤非生物因子关系研究[D]. 兰州: 甘肃农业大学, 2015.
	[Lu Xiaomei. Relationship of heterogeneity and non-biological soil factors in soil microbial of Haloxylon ammodendron from Minqin[D]. Lanzhou: Gansu Agricultural University, 2015.]
[13]	吕星宇, 张志山. 固沙植被区土壤质地与土壤微生物数量的关系[J]. 中国沙漠, 2019, 39(5): 71-79.
	[Lü Xingyu, Zhang Zhishan. Recovery of soil microbe quantities dependent on fine particle contents after establishment of sand-fixing revegetation in desert region[J]. Journal of Desert Research, 2019, 39(5): 71-79.]
[14]	张克存, 安志山, 何明珠, 等. 乌海至玛沁高速公路中卫段风沙环境特征及沙害防治[J]. 干旱区地理, 2021, 44(4): 983-991.
	[Zhang Kecun, An Zhishan, He Mingzhu, et al. Aeolian sand environments and disaster prevention along Zhongwei section of the Wuhai-Maqin Highway[J]. Arid Land Geography, 2021, 44(4): 983-991.]
[15]	许华, 何明珠, 唐亮, 等. 荒漠土壤微生物量碳、氮变化对降水的响应[J]. 生态学报, 2020, 40(4): 1295-1304.
	[Xu Hua, He Mingzhu, Tang Liang, et al. Responses of changes of microbial biomass carbon and nitrogen to precipitation in desert soil[J]. Acta Ecologica Sinica, 2020, 40(4): 1295-1304.]
[16]	李宇君, 张磊. 基于沙地指数模型的沙地监测方法[J]. 地球信息科学学报, 2021, 23(4): 680-691. doi: 10.12082/dqxxkx.2021.200161
	[Li Yujun, Zhang Lei. Sandy land monitoring method based on classification index model[J]. Journal of Geo-information Science, 2021, 23(4): 680-691.] doi: 10.12082/dqxxkx.2021.200161
[17]	李婷, 张威, 刘光琇, 等. 荒漠土壤微生物群落结构特征研究进展[J]. 中国沙漠, 2018, 38(2): 329-338.
	[Li Ting, Zhang Wei, Liu Guangxiu, et al. Advances in the study of microbial ecology in desert soil[J]. Journal of Desert Research, 2018, 38(2): 329-338.]
[18]	李帅, 董雷, 李文均. 荒漠放线菌的资源分布、多样性及分离方法研究进展[J]. 生物资源, 2020, 42(5): 481-493.
	[Li Shuai, Dong Lei, Li Wenjun. Distribution, diversity and isolation methods of Actinobacteria in desert[J]. Biotic Resources, 2020, 42(5): 481-493.]
[19]	邵玉琴, 赵吉, 朱艳华, 等. 科尔沁不同类型沙地土壤微生物类群的研究[J]. 内蒙古大学学报(自然科学版), 2007(6): 678-682.
	[Shao Yuqin, Zhao Ji, Zhu Yanhua, et al. Study on soil microorganism groups of different types of sandy land in Korqin Inner Mongolia[J]. Acta Scientiarum Naturalium Universitatis Neimongol (Natural Science Edition), 2007(6): 678-682.]
[20]	靳新影, 张肖冲, 金多, 等. 腾格里沙漠东南缘不同生物土壤结皮细菌多样性及其季节动态特征[J]. 生物多样性, 2020, 28(6): 718-726. doi: 10.17520/biods.2019298
	[Jin Xinying, Zhang Xiaochong, Jin Duo, et al. Diversity and seasonal dynamics of bacteria among different biological soil crusts in the southeast Tengger Desert[J]. Biodiversity Science, 2020, 28(6): 718-726.] doi: 10.17520/biods.2019298
[21]	孙沛沛, 钱朝菊, 尹晓月, 等. 半干旱区沙地蒿类植被建成对土壤细菌的影响[J]. 生态学报, 2020, 40(16): 5783-5792.
	[Sun Peipei, Qian Chaoju, Yin Xiaoyue, et al. Effects of artemisia vegetation built on soil bacteria in semi-arid sandy land[J]. Acta Ecologica Sinica, 2020, 40(16): 5783-5792.]
[22]	王彦武, 柴强, 欧阳雪芝, 等. 民勤绿洲荒漠过渡带固沙林土壤微生物数量和酶活性研究[J]. 干旱区地理, 2016, 39(1): 104-111.
	[Wang Yanwu, Chai Qiang, Ouyang Xuezhi, et al. Soil microbial populations and enzyme activity under different sand-fixation forest in oasis-desert ecotone of Minqin Desert[J]. Arid Land Geography, 2016, 39(1): 104-111.]
[23]	高东, 何霞红. 生物多样性与生态系统稳定性研究进展[J]. 生态学杂志, 2010, 29(12): 2507-2513.
	[Gao Dong, He Xiahong. Research advances on biodiversity and ecosystem stability[J]. Chinese Journal of Ecology, 2010, 29(12): 2507-2513.]
[24]	李靖宇, 张琇, 孙敏, 等. 腾格里沙漠沙坡头地区土壤微生物多样性分析[J]. 生态与农村环境学报, 2016, 32(5): 780-787.
	[Li Jingyu, Zhang Xiu, Sun Min, et al. Analysis of soil microbial diversity in Shapotou area of Tengger Desert[J]. Journal of Ecology and Rural Environment, 2016, 32(5): 780-787.]
[25]	戴雅婷, 闫志坚, 解继红, 等. 基于高通量测序的两种植被恢复类型根际土壤细菌多样性研究[J]. 土壤学报, 2017, 54(3): 735-748.
	[Dai Yating, Yan Zhijian, Xie Jihong, et al. Soil bacteria diversity in rhizosphere under two types of vegetation restoration based on high throughput sequencing[J]. Acta Pedologica Sinica, 2017, 54(3): 735-748.]
[26]	王彦武, 罗玲, 张峰, 等. 河西绿洲荒漠过渡带梭梭林土壤保育效应[J]. 土壤学报, 2019, 56(3): 749-762.
	[Wang Yanwu, Luo Ling, Zhang Feng, et al. Soil conservation effects of Haloxylon ammodendron bushes in Hexi Oasis Desert Ecotone[J]. Acta Pedologica Sinica, 2019, 56(3): 749-762.]
[27]	杨贵森, 吕星宇, 虎瑞, 等. 腾格里沙漠固沙植被区微生境土壤微生物功能群时空格局[J]. 土壤学报, 2022, 59(2): 580-590.
	[Yang Guisen, Lü Xingyu, Hu Rui, et al. Spatial and temporal patterns of soil microbial functional groups in different microhabitats in sand-fixing revegetation area in the Tengger Desert[J]. Acta Pedologica Sinica. 2022, 59(2): 580-590.]
[28]	杨瑞红, 赵成义, 王新军, 等. 梭梭和柽柳土壤微生物多样性初步分析[J]. 土壤, 2016, 48(6): 1120-1130.
	[Yang Ruihong, Zhao Chengyi, Wang Xinjun, et al. Phylogenetic diversity preliminary analysis of Haloxylon ammodendron and Tamarix ramosissima soil bacteria[J]. Soils, 2016, 48(6): 1120-1130.]
[29]	舒维花, 蒋齐, 王占军, 等. 植被恢复对土壤微生物的影响[J]. 农业科学研究, 2012, 33(1): 73-79.
	[Shu Weihua, Jiang Qi, Wang Zhanjun, et al. Research advances about the effects of vegetation recovery on soil microorganism[J]. Journal of Agricultural Sciences, 2012, 33(1): 73-79.]
[30]	陈孟立, 曾全超, 黄懿梅, 等. 黄土丘陵区退耕还林还草对土壤细菌群落结构的影响[J]. 环境科学, 2018, 39(4): 1824-1832.
	[Chen Mengli, Zeng Quanchao, Huang Yimei, et al. Effects of the program of converting farmland into forest or grassland on soil bacterial community structure in Loess Hilly Region[J]. Environmental Science, 2018, 39(4): 1824-1832.]
[31]	李媛媛, 彭梦文, 党寒利, 等. 塔里木河下游胡杨根际土壤细菌群落多样性分析[J]. 干旱区地理, 2021, 44(3): 750-758.
	[Li Yuanyuan, Peng Mengwen, Dang Hanli, et al. Bacterial communities diversity of Populus euphratica rhizospheric soil in the lower reaches of Tarim River[J]. Arid Land Geography, 2021, 44(3): 750-758.]
[32]	蒲洁, 齐雁冰, 王茵茵, 等. 农牧交错带不同植被群落对土壤微生物量碳氮磷的影响[J]. 干旱地区农业研究, 2015, 33(4): 279-285.
	[Pu Jie, Qi Yanbing, Wang Yinyin, et al. Effects of different plant communities on soil microbial biomass carbon, nitrogen and phosphorus in the agro-pastoral transitional zone of northern Shaanxi Province[J]. Agricultural Research in the Arid Areas, 2015, 33(4): 279-285.]
[33]	韩晓姝. 科尔沁沙地不同固沙群落土壤微生物多样性研究[D]. 沈阳: 东北大学, 2010.
	[Han Xiaoshu. Soil microbial diversity of different sand-fixation plantations in Horqin Sandy Land[D]. Shenyang: Northeastern University, 2010.]

Metrics

Viewed

Full text

520

HTML			PDF

Just accepted	Online first	Issue	Just accepted	Online first	Issue
0	0	1	0	0	519

From	Others	local

Times	362	158
Rate	70%	30%

Abstract

317

Just accepted	Online first	Issue

0	0	317

From	Others	local

Times	312	5
Rate	98%	2%

Cited

Web of Science	Crossref	ScienceDirect	Search for Citations in Google Scholar >>


This page requires you have already subscribed to WoS.

Shared

菌属	植被重建区/%	流沙区/%
异常球菌属（Deinococcus）	0.21	2.44
栖水菌属（Enhydrobacter）	0.04	0.72
烟草黄色土源菌属（Flavisolibacter）	0.44	1.00
微红微球菌属（Rubellimicrobium）	1.02	0.80
甲基柔膜菌属（Methylotenera）	1.24	0.81
壤霉菌属（Agromyces）	1.00	0.41
微枝型杆菌属（Microvirga）	1.32	0.72
根瘤菌属（Rhizobium）	0.85	0.01
大理石雕菌属（Marmoricola）	1.27	0.41
诺卡氏菌属（Nocardioides）	7.19	1.33

区域	土层/cm	平均长度/bp	物种数	距离	覆盖度	Chao1指数	Ace指数	Simpson指数	Shannon指数
植被重建区	0~5	411	1815	0.03	0.985	2886	2423	0.980	7.878
	5~10	409	1576	0.03	0.990	1812	1876	0.734	5.033
	10~20	411	1589	0.03	0.992	1750	1778	0.865	6.248
	20~50	411	1073	0.03	0.991	1451	1434	0.744	4.041
	50~100	415	1956	0.03	0.991	2093	2177	0.960	8.100
	平均	411	1602	0.03	0.990	1998	1938	0.857	6.260
流沙区	0~5	412	1908	0.03	0.991	2101	2137	0.986	8.207
	5~10	413	1547	0.03	0.991	1758	1807	0.893	6.212
	10~20	410	1164	0.03	0.988	1730	1675	0.352	2.309
	20~50	410	1362	0.03	0.991	1533	1644	0.549	3.599
	50~100	410	1398	0.03	0.988	1771	1830	0.521	3.330
	平均	411	1476	0.03	0.990	1779	1819	0.660	4.731

区域	土层/cm	pH	EC/μS·cm^-1	Salt/ng·L^-1	MBP/mg·kg^-1	MBC/mg·kg^-1	MBN/mg·kg^-1	MBC:MBN	Eh/mV
植被重建区	0~5	8.21	40.80	0.20	0.18	85.47	0.95	89.95	-63.1
	5~10	8.15	43.00	0.20	0.26	144.19	0.92	156.92	-78.0
	10~20	8.19	41.70	0.20	0.27	392.87	1.16	338.38	-84.0
	20~50	8.13	45.20	0.20	0.03	25.26	1.74	14.54	-89.0
	50~100	8.16	41.80	0.20	0.20	583.18	2.32	251.83	-95.9
流沙区	0~5	7.92	79.60	0.40	0.13	11.19	2.74	4.09	-67.5
	5~10	8.43	78.80	0.40	0.14	63.84	2.32	27.54	-66.2
	10~20	8.41	81.70	0.40	0.05	12.02	1.97	6.09	-66.2
	20~50	8.53	78.30	0.30	0.37	13.68	1.89	7.25	-63.7
	50~100	8.69	74.30	0.30	0.20	90.00	1.35	66.70	-64.0

Effect of preliminary vegetation reconstruction on soil microorganism community structure in arid desert area

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 33

Related Articles 0

Metrics

Comments

Recommended 0