羽毛针禾(Stipagrostis pennata)根鞘暗色丝孢菌降解功能的初步探究

干旱区地理 ›› 2017, Vol. 40 ›› Issue (4): 817-823.

羽毛针禾(Stipagrostis pennata)根鞘暗色丝孢菌降解功能的初步探究

陈洁作^1,2, 王慧敏^1,2, 郭建伟^1,3, 胡文革², 吴楠¹, 张元明¹

1. 中国科学院新疆生态与地理研究所, 中国科学院干旱区生物地理与生物资源重点实验室, 新疆乌鲁木齐 830011;
2. 石河子大学, 生命科学学院, 新疆石河子 832000;
3. 云南红河学院, 云南省农作物优质高效栽培与安全控制重点实验室, 云南蒙自 661100

收稿日期:2017-02-20 修回日期:2017-05-11 出版日期:2017-07-25
通讯作者: 张元明.Email:zhangym@ms.xjb.ac.cn
作者简介:陈洁作(1995-),女,新疆昌吉人,学士学位,从事荒漠植物-微生物互作研究.Email:18299083607@163.com
基金资助:
新疆维吾尔自治区杰出青年科技人才培养项目真菌菌丝桥在荒漠先锋植物羽毛针禾固沙区氮素转运中的作用（2013711013）；土壤微生物与荒漠先锋植物羽毛针禾根鞘形成的关系（编号：41271281）

Degradation function of dematiaceous hyphomycetes isolated from rhizosheath of desert plant Stipagrostis pennata

CHEN Jie-zuo^1,2, WANG Hui-ming^1,2, GUO Jian-wei^1,3, HU Wen-ge², WU Nan¹, ZHANG Yuan-ming¹

1. Key Laboratory of Biogeography and Bioresource in Arid land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, Xinjiang, China;
2. College of Life of Science, Shihezi university, Xinjiang, Shihezi 832000, Xinjiang, China;
3. Key Laboratory for Crop High Quality Cultivation and Security Control of Yunnan Province, College of Life Science and Technology, Honghe University, Mengzi 661100, Yunnan, China

Received:2017-02-20 Revised:2017-05-11 Online:2017-07-25

摘要/Abstract

摘要： 羽毛针禾（Stipagrostis pennata）因其特殊的根鞘结构而成为新疆北部古尔班通古特沙漠中流动和半流动沙丘上的先锋禾草和优良固沙植物。通过多种方法对5株供试暗色丝孢菌的降解功能进行初步探究，以期为高效降解真菌的选育工作提供理论依据。结果表明：（1）通过羧甲基纤维素钠（CMC-Na）水解圈测定法挑选产纤维素酶的菌株，据透明圈直径与菌落直径的比值判断其产酶能力的大小，除0103菌株外其余株菌均具分解纤维素的能力，0104菌株Dp值高达1.70，其余菌株依次为0304 > 0105 > 0204。（2）采用滤纸条降解法、失重法，对产纤维素酶的菌株进行降解纤维素功能的验证，其中0104菌株降解能力较为明显，其滤纸失重率高达55.72%。（3）在以聚乙烯为唯一碳源的琼脂培养基培养上述菌株，发现0103菌株具降解聚乙烯能力，光学显微镜下观测到被降解的单个聚乙烯膜片面积达3.75 mm²。由上可知，0104和0103菌株分别在降解纤维素及聚乙烯方面有潜在的应用价值，其研究结果也拓展了对根鞘微生物功能的认识，除细菌微生物菌肥之外，真菌资源的挖掘和利用也值得更进一步的关注。

关键词: 羽毛针禾, 根鞘, 暗色丝孢菌, 降解

Abstract: Stipagrostis pennata plant mainly distributed in Gurbantunggut Desert of the Junggar basin in the northern part of Tianshan Mountains,Xinjiang,China. It has an excellent drought resistance and sand fixation due to the special structure of rhizosheath. The present study for rhizosheath microorganism mainly concentrated in the nitrogen fixation strains,while the fungi function is poorly understood. Previous research has laid the foundation for the study of dematiaceous hyphomycetes from stipagrostis pennata rhizosheath,but their function and ecological significance still need further systematic research. Therefore,in this study,five dematiaceous hyphomycetes,which isolated from the rhizosheaths of S. pennata were chosen due to their strong stress resistance to investigate the production of cellulase enzyme and the ability of degradation of polyethylene. The results show that all selected strains except 0103 had the ability to produce cellulase. Strain 0104 showed the highest cellulase production by showing a clear halo zone around the colony on the selective medium,and the hydrolysis ability was as high as 1.70,followed by strains 0304,0105,and 0204. Moreover,the filter paper degradation and weight loss method confirmed the ability of strain 0104 for cellulose degradation,because the paper weight loss rate reached 55.72%. Cultivate five strains with polyethylene as the sole carbon source solid medium. strains 0103 was found to well degrade polyethylene. The cavity size produced by degradation of polyethylene was observed under the light microscope,and measure its size with Digimizer(3.75 mm²). Thus,0104 and 0103 strains showed potential in the degradation of cellulose and polyethylene. The functional fungi in the rhizosheath of S. pennata needs to be further developed and utilized.

Key words: Stipagrostis pennata, rhizosheath, dematiaceous hyphomycetes, degradation

中图分类号:

S182

陈洁作, 王慧敏, 郭建伟, 胡文革, 吴楠, 张元明. 羽毛针禾(Stipagrostis pennata)根鞘暗色丝孢菌降解功能的初步探究[J]. 干旱区地理, 2017, 40(4): 817-823.

CHEN Jie-zuo, WANG Hui-ming, GUO Jian-wei, HU Wen-ge, WU Nan, ZHANG Yuan-ming. Degradation function of dematiaceous hyphomycetes isolated from rhizosheath of desert plant Stipagrostis pennata[J]. , 2017, 40(4): 817-823.

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