土壤水分含量对虉草幼苗保护酶与渗透调节物质的影响

doi:10.12118/j.issn.1000-6060.2018.04.13

摘要/Abstract

摘要： 以虉草幼苗为试材，通过为期50 d的室内盆栽控水试验，按照土壤水分含量设置7个水分梯度：（10±0.5）%、（15±0.5）%、（20±0.5）%、（25±0.5）%、（30±0.5）%、（40±0.5）%（土壤水分饱和）和51%（淹水2 cm），研究了不同土壤水分含量对植物的抗氧化酶活性、丙二醛含量、可溶性蛋白含量的影响。结果表明：（1）土壤水分含量在20%~35%之间时SOD （超氧化物歧化酶）活性较高，低于20%和高于35%时，SOD活性下降，且在不同土壤水分含量下试验50 d的SOD活性均大于试验25 d的。（2） CAT（过氧化氢酶）与POD （过氧化物酶）活性达极显著相关p<0.01，均随土壤水分含量的增加呈先大幅下降后平稳再小幅上升的趋势。（3） MDA （丙二醛）含量与SOD活性趋势相反，随土壤水分含量的增加不断下降，在试验25 d时淹水组略有上升。（4）可溶性蛋白在20%~40%之间时含量较高，植株生长状况较好，在干旱和淹水胁迫条件下可溶性蛋白含量呈下降趋势。由此可见：当虉草幼苗受到干旱等胁迫时，虉草幼苗会开启抗氧化酶系统以保护植株组织，可长时间的胁迫或者胁迫超过其耐受范围（土壤水分含量小于20%或处于土壤水分过饱和淹水条件）就会不同程度的破坏植物的防御系统从而影响其生长发育。

关键词: 虉草, 抗氧化酶系统, 丙二醛, 可溶性蛋白, 土壤水分含量

Abstract: Soil moisture is an important environmental factor that affects plant growth and physiological metabolism.Under water stress conditions, plants can start the protective enzyme/antioxidant enzyme system and adjust the content of osmotic adjustment substances to respond to the environment.To study the P. arundinaceas' responding mechanism of antioxidant enzyme defencing system to different soil moisture,we selected P. arundinaceas seedlings in good growth condition with well preserved rhizome and consistent germination as test materials.Through 50 days indoor potted water control experiment,we set up seven water gradients of (10±0.5)%、(15±0.5)%、(20±0.5)%、(25±0.5)%、(30±0.5)%、(40±0.5)% (saturated soil moisture) and 51% (2 cm flooded) based on the volumetric water content.The soil moisture content was controlled by manual watering method.It was measured with a HH2 soil moisture meter every day at 20:00 PM,calculated the required moisture volume based on the soil moisture status and quantified the supplement to the set value.The effects of different soil moisture content on malondialdehyde (MDA),superoxide dismutase(SOD),peroxidase(POD),catalase(CAT) and soluble protein content were studied using nitroblue tetrazolium method to measure SOD,guaiacol method to measure POD,CAT was measured by potassium permanganate titration method to measure CAT,Glucosinolates barbituric acid method to measure MDA and Coomassie brilliant blue G-250 dyeing method to measure soluble protein.The results showed as follows:(1) The activity of SOD was higher when the soil moisture content was between 20% and 35%,and when the soil moisture content was below 20% or above 35%,the activity of SOD was decreased.The activities of SOD in 50 days were higher than those in 25 days of the experiment under different soil water content. (2) The activities of CAT and POD were extremely significantly correlated with p<0.01,both of which had a trend with a sharp drop at the beginning,and then becoming steady,then going up slightly with the increase of soil moisture content. (3) The content of MDA was opposite to the trend of SOD,and it was decreased with the increase of soil moisture content.The C7 group (flooding 2 cm) was increased slightly in 25 days of the experiment. (4) The content of soluble protein was higher with the value between 20% and 40%,and the plants were growing well.The soluble protein showed a drop trend under drought or flooding stress conditions.So it can be seen that when the P. arundinaceas seedling was exposed to drought or other stress,it will trigger the antioxidant enzyme system to protect the plant tissue,and the antioxidant enzyme defense system would work through a series of antioxidant protection mechanism to increase plant stress resistance ability,reduce or eliminate the damages to the plants,but the long-term stress or the stress which beyond the plant's tolerance (the soil moisture content is less than 20% or the soil moisture content is oversaturated) will destroy the plant's defense system in various degrees thus affecting the plant's growth and development.

Key words: P. arundinacea, antioxidase, Malondialdehyde, soluble protein, soil water content

中图分类号:

S152.7

曹昀, 纪欣圣, 国志昌, 吴海英. 土壤水分含量对虉草幼苗保护酶与渗透调节物质的影响[J]. 干旱区地理, 2018, 41(4): 780-785.

CAO Yun, JI Xin-sheng, GUO Zhi-chang, WU Hai-ying. Effects of soil moisture content on antioxidase and osmoregulation substrate content of Phalaris arundinacea seedlings[J]. 干旱区地理, 2018, 41(4): 780-785.

参考文献

[1] 张永亮,骆秀梅.虉草的研究进展[J].草地学报,2009,16(6):659-666.[ZHANG Yongliang,LUO Xiumei.Research progress of reed canarygrass[J].Acta Agrestia Sinica,2009,16(6):659-666.]
[2] 张永亮,张浩,徐金波,等.虉草人工草地的生长动态研究[J].中国草地学报,2012,34(3):21-27.[ZHANG Yongliang,ZHANG Hao,XU Jinbo,et al.The growth dynamic research of reed canarygrass artificial grassland[J].Chinese Journal of Grassland,2012,34(3):21-27.]
[3] SAHRAMAA M.Evaluation of reed canary grass for different end-uses and in breeding[J].Agricultural & Food Science,2003,23(3-4):227-241.
[4] 国志昌,曹昀,陈冰祥,等.根茎萌发与幼苗期的虉草水分生态幅[J].广西植物,2016,36(8):1000-1007.[GUO Zheng chang,CAO Yun,CHEN Bingxiang,et al.Water ecological amplitude of Phalaris arundinacea at rhizomatic germination and seedling stage[J].Guihaia,2016,36(8):1000-1007.]
[5] 聂微微,张永亮,任秀珍,等.盐碱胁迫对虉草幼苗生理特性的影响[J].内蒙古民族大学学报:自然科学版,2010,25(2):175-177.[NIE Weiwie,ZHANG Yongliang,REN Xiuzhen,et al.Effect saline-alkali stress on physiological characteristics of Phalaris arundinacea[J].Journal of Inner Mongolia University for Nationalities,2010,25(2):175-177.]
[6] 张永亮.氮磷钾肥对盐胁迫下虉草离子吸收与分布的影响[C]//中国草学会.中国草学会2013学术年会论文集.中国草学会,2013:6.[ZHANG Yongliang.Effects of nitrogen phosphorus potassium fertilization on the absorption and distribution of Ions in reed canarygrass under salt stress[C]//Chinese Herb Society.Chinese herb society proceedings of 2013 academic conference.Chinese Herb Society,2013:6.]
[7] 李萍萍,陈歆,付为国,等.虉草光合作用日变化及其与环境因子的关系[J].生态学杂志,2006,25(10):1157-1160.[LI Pingping,CHEN Xin,FU Weiguo,et al.Diurnal change of Phalaris arundinacea photosynthetic characteristics and its relations with environmental factors[J].Chinese Journal of Ecology,2006,25(10):1157-1160.]
[8] 张小晶,宗仁旭,游明鸿,等.灌浆期虉草不同位叶对光照强度响应能力及光合贡献率[J].草业科学,2016,33(5):942-948.[ZHANG Xiaojing,ZONG Renxu,YOU Minghong,et al.Photosynthetic contribution rate and response of Phalaris arundinacca leaves at the different position in filling stage to light intensity[J].Pratacultural Science,2016,33(5):942-948.]
[9] 宋家壮,李萍萍,付为国,等.水分胁迫及复水对虉草生理生化特性的影响[J].草业学报,2012,21(2):62-69.[SONG Jiazhuang,LI Pingping,FU Weiguo,et al.Effect of water stress and rewatering on the physiological and biochemical characteristics of Phalaris arundinacea[J].Acta Prataculturae Sinica,2012,21(2):62-69.]
[10] 王学奎.植物生理生化实验原理和技术[M].二版.北京:高等教育出版社,2006:190-192.[WANG Xuekui.Plant physiological and biochemical experiment principle and technology[M].2nd edition.Beijing:Higher Education Press,2006:190-192.]
[11] 马旭俊,朱大海.植物超氧化物歧化酶(SOD)的研究进展[J].遗传,2003,25(2):225-231.[MA Xujun,ZHU Dahai.Functional roles of the plant superoxide dismutase[J].Hereditas(Beijing),2003,25(2):225-231.]
[12] 崔江慧,李霄,常金华,等.PEG模拟干旱胁迫对高粱幼苗生理特性的影响[J].中国农学通报,2011,27(9):160-165.[CUI Jianghui,LI Xiao,CHANG Jinhua,et al.Biochemical characteristics in sorghum seedlings under PEG simulated drought stress[J].Chinese Agricultural Science Bulletin,2011,27(9):160-165.]
[13] 姜慧芳,任小平.干旱胁迫对花生叶片SOD活性和蛋白质的影响[J].作物学报,2004,30(2):169-174.[JIANG Huifang,REN Xiaoping.The effect on SOD activity and protein content in groundnut leaves by drought stress[J].Acta Agronomica Sinica,2004,30(2):169-174.]
[14] 张永峰,殷波.混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响[J].草业学报,2009,18(1):46-50.[ZHANG Yongfeng,YIN Bo.Influences of salt and alkali mixed stresses on antioxidative activity and MDA content of Medicago sativa at seedling stage[J].Acta Prataculturae Sinica,2009,18(1):46-50.]
[15] 籍越,滕开琼.Na₂CO₃胁迫对不同草坪品种O₂产生及SOD活性的影响[J].河南农业大学学报,2000,34(2):177-179.[JI Yue,TENG Kaiqiong.The effect of Na₂CO₃ on O₂-produciton and SOD activity in various lawn breeds[J].Journal of Henan Agricultural University,2000,34(2):177-179.]
[16] 刘晓静,柳小妮.多效唑和烯效唑对草地早熟禾一些生化指标及其抗性的影响[J].草业学报,2006,15(2):48-53.[LIU Xiaojing,LIU Xiaoni.Effects of paclobutrazol and uniconazole on some physiological and biochemical indices and the resistance of Poa pratensis[J].Acta Prataculturae Sinica,2006,15(2):48-53.]
[17] 刘建新,胡浩斌,赵国林,等.多裂骆驼蓬中生物碱类物质对玉米幼苗生长及某些生理特性的影响[J].草业学报,2007,16(1):75-80.[LIU Jianxin,HU Haobin,ZHAO Guolin,et al.The physiological characteristics effects of Z.mays seeds at different concentrations of alkaloid extract from P.multisectum[J].Acta Prataculturae Sinica,2007,16(1):75-80.]
[18] 李雁博.须芒草、虉草和柳枝稷对干旱和盐耦合胁迫的生理响应[D].杨凌:西北农林科技大学,2014.[LI Yanbo.Physiological coupling response to drought and salinity stress in bluestem,reed canarygrass and switchgrass[D].Yangling:Northwest Agriculture & Forestry University,2014.]
[19] 蔡仕珍,潘远智,陈其兵,等. 低温胁迫对花叶细辛生理生化及生长的影响[J].草业学报,2010,19(1):95-102.[CAI Shizhen,PAN Yuanzhi,CHEN Qibing,et al.Effects of low temperatures on physiological and biochemical indexes and growth of Asarum splendens[J].Acta Prataculturae Sinica,2010,19(1):95-102.]
[20] 汪月霞,孙国荣,王建波,等.NaCl胁迫下星星草幼苗MDA含量与膜透性及叶绿素荧光参数之间的关系[J].生态学报,2006,26(1):122-129.[WANG Yuexia,SUN Guorong,WANG Jianbo,et al.Relationships among MDA content,plasma membrane permeability and the chlorophyll fluorescence parameters of Puccinellia tenuiflora seedlings under NaCl stress[J].Acta Ecologica Sinica,2006,26(1):122-129.]
[21] BERRY J A,DOWNTON W J S.Environmental regulation of photosynthesis[M]//GOVIND J E ed.Photosynthesis(Vol.Ⅱ).New York:Academic Press,1982:294-306.
[22] 米海莉,许兴,李树华,等.水分胁迫对牛心朴子、甘草叶片色素、可溶性糖、淀粉含量及碳氮比的影响[J].西北植物学报,2004,24(10):1816-1821.[MI Haili,XU Xing,LI Shuhua,et al.Effects of soil water stress on contents of chlorophyll,soluble sugar,starch,C/N of two desert plants (Cynanchum komarovii and Glycyrrhiza uralensis)[J].Acta Bot Boreal Occident Sin,2004,24(10):1816-1821.]
[23] 董伊晨,刘悦秋.土壤水分对异株荨麻保护酶和渗透调节物质的影响及其与叶片光合和生物量的相关性[J].生态学报,2009,29(6):2845-2851.[DONG Yichen,LIU Yueqiu.Soil water influences on protective enzymes and osmolytes of Urtica dioica and their correlations with leaf photosynthesis and biomass[J].Acta Ecologica Sinica,2009,29(6):2845-2851.]
[24] CHEN S Y.Membrane-lipid peroxidation and plant stress[J].Chinese Bulletin of Botany,1989,6(4)211-217.