收稿日期: 2021-02-04
修回日期: 2021-02-27
网络出版日期: 2021-06-01
基金资助
国家自然科学基金地区项目(31560177)
Bacterial communities diversity of Populus euphratica rhizospheric soil in the lower reaches of Tarim River
Received date: 2021-02-04
Revised date: 2021-02-27
Online published: 2021-06-01
采用高通量测序技术,对塔里木河下游不同生长时期（幼龄期、中壮期、过熟期、衰亡期）胡杨根际土壤细菌进行测序,结合典范对应分析（CCA）与Spearman相关性分析,探讨细菌群落组成与环境因子的相关性。结果表明：（1） 土壤样品共获得7287个操作分类单元（OTUs）,经过对比鉴定共得到73门,165纲,339目,454科,651属和205种。（2） 胡杨根际土壤细菌群落丰富度和多样性随生长时期表现为先增加后降低的趋势,而不同生长时期间无显著差异。（3） 胡杨根际细菌群落主要的优势细菌门为变形菌门（Proteobacteria）、unidentified_Bacteria、Halobacterota,优势细菌属为海杆菌属（Marinobacter）、嗜盐单胞菌属（Halomonas）、Woeseia,相较于门分类学水平,细菌群落组成在属水平上存在较大差异,不同生长时期胡杨根际细菌群落的优势菌属不同。（4） 不同生长时期胡杨根际土壤细菌群落组成可分为两大类,中壮期与衰亡期的土壤样品聚为一类,幼龄期与过熟期的土壤样品聚为一类。（5） CCA分析表明土壤含水量、全钾、总盐、pH是显著影响胡杨根际土壤细菌群落组成的环境因子（P<0.05）。研究结果为丰富干旱区根际微生物的研究、探讨干旱区植物-微生物之间的相互作用提供科学依据。
李媛媛,彭梦文,党寒利,姜梦,庄丽,李桂芳 . 塔里木河下游胡杨根际土壤细菌群落多样性分析[J]. 干旱区地理, 2021 , 44(3) : 750 -758 . DOI: 10.12118/j.issn.1000–6060.2021.03.17
Rhizosphere bacteria play an essential role in promoting plant growth and protecting plant health. In this study, we use the high throughput sequencing to analyze rhizosphere bacteria at different developmental periods of Populus euphratica (the four periods are young, medium, overripe, and decline periods) in the lower reaches of Tarim River in Xinjiang, China. Canonical correspondence and Spearman correlation analyses were used to investigate the correlation between bacterial community and environmental factors. The results showed that (1) out of the soil samples collected from the soils under the four developmental periods of P. euphratica, 98028 effective sequences and 7287 operational taxonomic units (OTUs) were obtained, the OTUs numbers of the four periods were 3701, 4543, 4297, and 3710, respectively. From comparative identification, 73 phyla, 165 classes, 339 orders, 454 families, 651 genera, and 205 species were obtained with the development of P. euphratica. (2) Alpha diversity analysis showed that the bacterial community diversity was the highest at the overripe period (Shannon and Simpson index), the bacterial community abundance was the highest at the medium period (Chao1 and ACE index), the abundance and diversity of the rhizosphere bacterial community showed a trend of first increasing and then decreasing; however, there was no significant difference in bacterial abundance and diversity in rhizosphere soil at different developmental periods. (3) Proteobacteria, unidentified_Bacteria, and Halobacterota were the dominant bacteria at the phylum level in the rhizosphere of P. euphratica, whereas Marinobacter, Halomonas, and Woeseia were the dominant bacteria at the genus level. Compared with the phylum level, the bacterial community composition of soil samples was significantly different at the genus level. The dominant bacterial genera of rhizosphere bacterial community of P. euphratica were different in different developmental periods; Marinobacter, Halomonas, and Woeseia were, respectively, the optimal bacteria genus in the young, medium/overripe, and decline periods. (4) As shown in the cluster analysis, the bacterial communities of all soils were divided into two groups: the medium and decline periods were clustered into one group, and the young and overripe periods were clustered into another group. (5) Canonical correspondence analysis showed that the soil water content, total potassium, total salt, and soil pH were the main environmental factors influencing the bacterial community composition in the rhizosphere soil of P. euphratica (P<0.05). The results revealed the composition of rhizosphere bacterial communities in different periods of P. euphratica and the main environmental factors affecting the community composition. The findings in this study may provide a scientific basis for the study of rhizosphere microorganisms and the interaction between plants and microorganisms in arid areas.
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