民勤绿洲荒漠过渡带固沙林土壤微生物数量和酶活性研究

干旱区地理 ›› 2016, Vol. 39 ›› Issue (1): 104-111.

民勤绿洲荒漠过渡带固沙林土壤微生物数量和酶活性研究

王彦武^1,2, 柴强^1,3, 欧阳雪芝², 罗玲⁴

1. 甘肃农业大学资源环境学院, 甘肃兰州 730070;
2. 甘肃省水土保持科学研究所, 甘肃兰州 730020;
3. 甘肃农业大学农学院, 甘肃兰州 730070;
4. 河南理工大学资源环境学院, 河南焦作 454000

收稿日期:2015-08-25 修回日期:2015-11-10 出版日期:2016-01-25
通讯作者: 柴强(1972-),男,甘肃武威人,博士,教授,主要从事旱地与绿洲农作制及生态环境方面的研究.Email:Chaiq@gsau.edu.cn
作者简介:王彦武(1982-),男,甘肃会宁人,博士研究生,工程师,主要从事水土保持与荒漠化防治方面的研究.Email:wang_yanwu@163.com
基金资助:
甘肃省自然科学基金项目(1308RJZA237);甘肃省青年科技基金项目(1506RJYA177)

Soil microbial populations and enzyme activities under different sand-fixation forests in oasis-desert ecotone of Minqin Desert

WANG Yan-wu^1,2, CHAI Qing^1,3, OUYANG Xue-Zhi², LUO Ling⁴

1 College of Resources and Environment, Gansu Agricultural University, Lanzhou 730070, Gansu, China;
2 Gansu Science Institute of Soil and Water Conservation, Lanzhou 730020, Gansu, China;
3 Agronomy College, Gansu Agricultural University, Lanzhou 730070, Gansu, China;
4 Institute of Resource and environment, Henan Polytechnic University, Jiaozuo 454000, Henan, China

Received:2015-08-25 Revised:2015-11-10 Online:2016-01-25

摘要/Abstract

摘要： 对民勤绿洲荒漠过渡带不同固沙林土壤微生物数量和土壤酶活性进行研究,以裸沙地为对照.结果表明:不同固沙林土壤微生物数量和土壤酶活性均存在差异,且均显著大于裸沙地;在0~60 cm土层中,土壤细菌、放线菌、真菌、过氧化氢酶、脲酶和磷酸酶以梭梭林地最大,分别是对照的2.29 倍、2.36 倍、10.17 倍、2.63 倍、1.65 倍和1.71 倍;土壤微生物数量及其酶活性有明显的垂直分布特征,梭梭林的放线菌、真菌和蔗糖酶活性均随着土层深度增加而减小;相关性研究表明,土壤细菌、真菌和放线菌与土壤过氧化氢酶、脲酶、磷酸酶呈极显著正相关关系,相关系数均达到了69.6%以上.随着民勤绿洲荒漠过渡带植被的恢复,不同固沙林对干旱瘠薄的沙漠环境均有一定的适应性,各固沙林土壤的生物性质不断得到改善.

关键词: 绿洲荒漠过渡带, 固沙林, 土壤微生物, 酶活性

Abstract: In vulnerable ecosystems,the soil microbial organization is intimately interacted with the vegetation. Studying the soil microbial characteristics in different sand-fixation forests is of great importance for ameliorating the forests soil condition and preventing desertification. Based on the wild typical sampling survey and lab analysis in Minqin Oasis-desert Ecotone,this study chose 4 main sand-fixation forests(Haloxylon forest,Tamarisk forest,Nitraria tangutorum forest,and Limonium sinense forest)to study the soil microbial population(including bacterias,fungus and actinomycetes)and soil enzymes activities(including catalases,urease,sucrase,alkaline phosphatase)in 0-20 cm,20-40 cm,40-60 cm soil layers,and analyzed the correlation between the soil microbial population and soil enzyme. Results showed that: soil microbial population and enzyme activities differed significantly in different sand-fixation forests. In the depth of 0-60 cm,the population of soil bacterias,actinomycetes, fungus,the activities of catalase,urease and phosphatase were the greatest in Haloxylon ammodendron forest which is 2.29,2.36,10.17,and 2.63,1.65 1.17 times of the control group,respectively. The soil microorganism distribution and enzyme activities had significantly vertical distribution characteristics. The population of soil actinomycetes,fungus and the activity of sucrase in Haloxylon forest were negatively correlated with the depth. Correlation analysis showed that the populations of soil bacteria,fungus and actinomycetes were significantly positive correlated with the activities of catalase,urease and phosphatase(correlation>69.6%). This study concluded that along with the restoration of Minqin Oasis-desert Ecotone,all sand-restoration forests were becoming more and more adaptive to the desert environment,populations of soil microbes and activities of enzymes increased and the biological characteristics were better than before. Considering the sand-fixation,soil-quality-improvement effect,and forest sustainable management,Haloxylon forest is the best choice to be planted widely around the ecotone because it can not only significantly improve the sand-fixing effects,but also improve the soil charateristics and microbe activities of the moving dunes.

Key words: Minqin Oasis-desert Ecotone, sand-fixation forests, soil microbial population, soil enzyme activities

中图分类号:

S158.3

王彦武, 柴强, 欧阳雪芝, 罗玲. 民勤绿洲荒漠过渡带固沙林土壤微生物数量和酶活性研究[J]. 干旱区地理, 2016, 39(1): 104-111.

WANG Yan-wu, CHAI Qing, OUYANG Xue-Zhi, LUO Ling. Soil microbial populations and enzyme activities under different sand-fixation forests in oasis-desert ecotone of Minqin Desert[J]. , 2016, 39(1): 104-111.

参考文献

[1] 汪媛燕,王立,满多清. 民勤绿洲荒漠过渡带群落特征及其物种多样性研究[J]. 四川农业大学学报,2014,32(4):355-361. [WANG Yuanyan,WANG Li,MAN Duoqing. Community characteristics and species diversity in the Minqin Oasis-desert Ecotone[J]. Journal of Sichuan Agricultural University,2014,32 (4):355-361.]

[2] 胥宝一,李得禄. 河西走廊荒漠化及其防治对策探讨[J]. 中国农学通报,2011,27(11):266-270.[XU Baoyi,LI Delu. Discussion on desertification combating and the countermeasures in Hexi Corridor[J]. Chinese Agricultural Science Bulletin,2011, 27(11):266-270.]

[3] 李玉强,赵学勇,刘新平,等. 樟子松固沙林土壤碳截存及土壤呼吸对干湿变化的响应[J]. 中国沙漠,2011,31(2):282-287. [LI Yuqiang,ZHAO Xueyong,LIU Xinping,et al. Soil carbon sequestration in sand-fixation plantation of Pinus sylvestris var. mongolica and response of soil respiration to drought and wet conditions[J]. Journal of Desert Research,2011,31(2):282-287.]

[4] 陈银萍,李玉强,赵学勇,等. 流动沙丘造林对土壤物理组分有机碳分配的影响[J]. 水土保持通报,2011,31(1):1-5.[CHEN Yinping,LI Yuqiang,ZHAO Xueyong,et al. Allocation of soil organic carbon in physical fractions as affected by plantation in mobile sand dune[J]. Bulletin of Soil and Water Conservation, 2011,31(1):1-5.]

[5] 刘任涛,柴永青,徐坤,等. 荒漠草原区柠条人工固沙林生长过程中地表植被—土壤的变化[J]. 应用生态学报,2012,23(11): 2955-2960.[LIU Rentao,CHAI Yongqing,XU Kun,et al. Variations of ground vegetation and soil properties during the growth process of artificial sand-fixing Caragana intermedia plantations in desert steppe[J]. Chinese Journal of Applied Ecology,2012, 23(11):2955-2960.]

[6] 杨文斌,郭建英,胡小龙,等. 低覆盖度行带式固沙林带间植被修复过程及其促进沙地逆转效果分析[J]. 中国沙漠,2012,32 (5):1291-1295.[YANG Wenbin,GUO Jianying,HU Xiaolong, et al. Vegetation restoration process and sand-reversal effect of low coverage sand-fixing forest belts[J]. Journal of Desert Research, 2012,32(5):1291-1295.]

[7] 姜丽娜,杨文斌,姚云峰,等. 行带式固沙林带间植被恢复及土壤养分变化研究[J]. 水土保持通报,2012,32(1):98-102. [JIANG Lina,YANG Wenbin,YAO Yunfeng,et al. Changes of inter-belt vegetation recovery and soil nutrients in belt-type sand fixation forest[J]. Bulletin of Soil andWater Conservation,2012, 32(1):98-102.]

[8] 姜丽娜,杨文斌,卢琦,等. 低覆盖度行带式固沙林对土壤及植被的修复效应[J]. 生态学报,2013,33(10):3192-3204.[JIANG Lina,YANG Wenbin,LU Qi,et al. The effect of low-covered sand-fixing forest belts on restoration of the soil and vegetation[J]. Acta Ecologica Sinica,2013,33(10):3192-3204.]

[9] 郭建英,杨文斌,胡小龙,等. 行带式防风固沙林带间植被和土壤修复效果分析[J]. 灌溉排水学报,2011,30(4):128-131. [GUO Jianying,YANG Wengbin,HU Xiaolong,et al. Analysis on the restoration effect of soil physical quality and vegetation in different width lines of wind break and sand fixation forest[J]. Journal of Irrigation and Drainage,2011,30(4):128-131.]

[10] 程丽娟,薛泉宏. 微生物学实验技术[M]. 西安:世界图书出版公司,2000:63-68.[CHENG Lijuan,XUE Quanhong. Microbiology laboratory technicians[M]. Xi'an:World Book Publishing Company,2000:63-68.]

[11] 杨文博. 微生物学实验[M]. 北京:化学工业出版社,2004:217-218.[YANG Wenbo. Laboratory experiments in microbiology[M]. Beijing:Chemical Industry Press,2004:217-218.]

[12] 关松荫. 土壤酶及其研究法[M]. 北京:中国农业出版社,1986: 256-312.[GUAN Songyin. Soil enzyme and their research method[M]. Beijing:China Agricultural Press,1986:256-312.]

[13] 徐恒,廖超英,李晓明,等. 榆林沙区人工固沙林土壤微生物生态分布特征及酶活性研究[J]. 西北农林科技大学学报(自然科学版),2008,36(12):135-141.[XU Heng,LIAO Chaoying, LI Xiaoming,et al. Soil microorganism and enzyme activities under different artificial sand-fixing vegetations in the sandy area of Yulin[J]. Journal of Northwest A&F University(Natural Science Edition),2008,36(12):135-141.]

[14] 单娜娜,潘伯荣,文启凯,等. 塔克拉玛干沙漠腹地人工绿地土壤微生物生态学特性研究[J]. 干旱区研究,2001,18(4):51-56.[SHAN Nana,PAN Borong,WEN Qikai,et al. Study on the ecological characteristics of soil-inhabiting microorganisms of the artificial vegetation in the hinterland of Taklamakan Desert[J]. Arid Zone Research,2001,18(4):51-56.]

[15] 王少昆,赵学勇,左小安,等. 科尔沁沙质草甸土壤微生物数量的垂直分布及季节动态[J]. 干旱区地理,2009,32(4):610-615.[WANG Shaokun,ZHAO Xueyong,ZUO Xiao'an,et al. Vertical distribution and seasonal dynamics of soil microbial number in sandy grassland of Horqin[J]. Arid Land Geography, 2009,32(4):610-615.]

[16] 许景伟,王卫东,李成. 不同类型黑松混交林土壤微生物、酶及其与土壤养分关系的研究[J]. 北京林业大学学报,2000,22 (1):51-55.[XU Jingwei,WANG Weidong,LI Cheng. The correlation among soil microorganism,enzyme and soil nutrient in different types of mixed stands of Pinus thunbergii[J]. Journal of Beijing Forestry University,2000,22(1):51-55.]

[17] 郭继勋,祝廷成. 羊草草原土壤微生物的数量和生物量[J]. 生态学报,1997,17(1):78-82.[GUO Jixun,ZHU Tingcheng. Study on numbers and biomass of soil microorganism in Aneurolepidium chinense grassland[J]. Acta Ecologica Sinica,1997,17 (1):78-82.]

[18] 刘子雄,朱天辉,张建. 林木根系分泌物与根际微生物研究进展[J]. 世界林业研究,2005,18(6):25-31.[LIU Zixiong,ZHU Tianhui,ZHANG Jian. Research advances in root exudates and rhizosphere microorganisms of forest trees[J]. World Forestry Research,2005,18(6):25-31.]

[19] 杨涛,徐慧,李慧,等. 樟子松人工林土壤养分、微生物及酶活性的研究[J]. 水土保持学报,2005,19(3):50-53.[YANG Tao, XU Hui,Li Hui,et al. Soil nutrient,microorganism and enzyme activity in Pinus sylvestris plantations[J]. Journal of Soil and Water Conservation,2005,19(3):50-53.]

[20] 陈立新. 施肥对落叶松人工林根际土壤生化活性的影响[J]. 水土保持学报,2003,17(3):133-136.[CHEN Lixin. Influence of fertilization on bio-chemical activeness of rhizosphere soil in larch plantations[J]. Journal of Soil and Water Conservation, 2003,17(3):133-136.]

[21] 吴秀臣,孙辉,杨万勤,等. 川西亚高山红桦幼苗土壤蔗糖酶活性对温度和大气二氧化碳浓度升高的响应[J]. 应用生态学报, 2007,18(6):1225-1230.[WU Xiuchen,SUN Hui,YANG Wanqin, et al. Responses of subalpine Betula albo-sinensis soil invertase activity to elevated atmospheric temperature and CO₂ concentration in western Sichuan[J]. Chinese Journal of Applied Ecology,2007,18(6):1225-1230.]

[22] 王冬梅,王春枝,韩晓日,等. 长期施肥对棕壤主要酶活性的影响[J]. 土壤通报,2006,37(2):263-268.[WANG Dongmei, WANG Chunzhi,HAN Xiaori,et al. Effects of long-term application of fertilizers on some enzymatic activities in brunisolic soil[J]. Chinese Journal of Soil Science,2006,37(2):263-268.]

[23] 邱莉萍,张兴昌,程积民. 坡向坡位和撂荒地对云雾山草地土壤酶活性的影响[J]. 草业学报,2007,16(1):87-93.[QIU Liping, ZHANG Xingchang,CHENG Jimin. Influence of slope direction, position and fallow measurement of soil enzymatic in grassland at Yunwu Mountain[J]. Acta Prataculturae Sinica, 2007,16(1):87-93.]

[24] 王少昆,赵学勇,张铜会,等. 造林对沙地土壤微生物的数量、生物量碳及酶活性的影响[J]. 中国沙漠,2013,33(2):529-535.[WANG Shaokun,ZHAO Xueyong,ZHANG Tonghui,et al. Effects of afforestation on the abundance,biomass carbon and enzymatic activities of soil microorganism in sandy dunes[J]. Journal of Desert Research,2013,33(2):529-535.]

[25] 马文文,姚拓,靳鹏,等. 荒漠草原2 种植物群落土壤微生物及土壤酶特征[J]. 中国沙漠,2014,34(1):176-183.[MA Wenwen, YAO Tuo,JIN Peng,et al. Characteristics of microorganisms and enzyme activity under two plant communities in desert steppe[J]. Journal of Desert Research,2014,34(1):176-183.]

[26] 赵之重. 土壤酶与土壤肥力关系的研究[J]. 青海大学学报(自然科学版),1998,16(3):24-29.[ZHAO Zhizhong. The Relationships between soil enzyme and soil fertility[J]. Journal of Qinghai University(Natural Science Edition),1998,16(3):24-29.]