关键词: 可培养细菌, ARDRA, 16S rDNA, 吉尔吉斯斯坦

Abstract: Cultured microorganism technologies are important ways to investigate environmental microbial diversties. The aim of this work was to explore the diversity of culturable bacteria isolated from Issyk Kul region in Kyrgyzstan. Soil samples collected from a hot spring（42°27′N，78°32′E） were isolated with 3 isolation media by dilution plate method. Based on morphological characteristics，32 bacteria strains were separated from the soil，and the culture characteristics were detected. It were showed that 18 strains were gram positive，and most of them could not grow beyond 15% NaCl concentration. Growth of pH general distributed between 4.5-8.5，simultaneously，optimum growth temperature was 40 ℃. Screened by selective media，15 strains produce protease，11 strains having amylase activity and obtained 26 esterase producing strains. By analysis of the restriction patterns of amplified 16S rDNA （ARDRA） with the enzyme Hae III and Hinf I，respectively，the isolates were clustered into 9 genotypes，then the representatives of each genotype were randomly chosen for the determination of 16S rDNA sequence. The phylogenetic analysis revealed that all of the isolates belonged to the following groups：Pseudomonas，Paenibacillus，Bacillus，Stenotrophomonas，Lysinibacillus，Lysobacter，Brevibacillus，Rhizobium，Proteus. Bacillus and Stenotrophomonas strains dominanted among the isolates，then followed by Brevibacillus members. One of the isolates showed less affilation with known taxon （<97% sequence similarity） and may represent novel taxa. The results suggest that the culturable bacteria are abundant and some unknown taxa can exist in Issyk Kul region.

Key words: culturable bacteria, ARDRA, 16S rDNA, diversity